Measurement

Part:BBa_J100278:Design

Designed by: Anna Buser   Group: Campbell M Lab   (2016-06-24)


tCLone Tet+PDB riboswitch with P14 and BD2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1051
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since there was only a 6 base difference between BD18 C Dog and BD2 C Dog, the PCR was used to insert in and amplify the new BD2 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.


Source

J100274

References